Shishir P S Chundawat’s Post

🧑 Leavers and Newcomers We have two M.Sc students and a PhD student starting at Indbio. Aleksandra Zagorska (Sweden; Supervisor: Elin Blick) will introduce genes involved in the violacein biosynthesis in yeast using CRISPR-technology, to generate a purple-colored yeast. Erik Berg (Sweden; Supervisor: Arne Peetermans) will target specific genetic markers associated with acetate utilization, integrating relevant genes, and quantifying outcomes through an acetate sensing biosensor. Yağmur Bektaş’s, (Turkey; Supervisors: Malgorzata Zboinska, PhD and Cecilia Geijer) doctoral project is titled "Resource efficient architectural renovation using a 3D printable material from underutilized biomass". ~ 🎉 Publications - PhD student Luca Torello Pianale partnered with fellow PhD student Fabio Caputo - who works with biomass pre-treatment and enzymatic hydrolysis- to validate the molecular tools he has developed. In their joint paper titled ‘Four ways of implementing robustness quantification in strain characterization’ (https://lnkd.in/gQdbe_6Z) (both supervised by Lisbeth Olsson), they employ intracellular parameters and macroscopic fluorescent biosensors to quantify the robustness of yeasts. ~ - Alumnus Andrea Seveso, PhD and coworkers show that carbohydrate esterases 15 family may also have other activities targeting pectins and other polysaccharides. Their work is now published in AEM: “Polysaccharide utilization loci from Bacteroidota encode CE15 enzymes with possible roles in cleaving pectin-lignin bonds.”: https://lnkd.in/g-pEpgNZ ~ When a claim was made by Zhao et al in their paper ‘Making the biochemical conversion of lignocellulose more robust’, that ‘…lignocellulose biorefinery is conceptually wrong’, two Indbio PI:s Carl Johan Franzén and Lisbeth Olsson and an alumna (Katja Salomon Johansen) submitted a letter to argue that Zhao et al’s claim has already been proved wrong by several companies: https://lnkd.in/gPwkwNs4 📆 Events 6 Feb - Luca Torello Pianale will defend his PhD thesis entitled ‘Tools and applications to assess yeast physiology and robustness in bioprocesses’ with Prof. Ton Van Maris as the faculty opponent. His thesis can be accessed here: https://lnkd.in/g3wYGXsC ~ 16 Apr – Cecilia Trivellin will defend her PhD thesis entitled ‘“Robustness quantification in yeast: A methodology to study phenotypic, evolutionary, and genomic aspects of microbial robustness” with Prof. Andreas Gombert as the faculty opponent. ~~~~ To know more about research activities at Indbio, LIFE, please visit 👉 www.chalmers.se/Indbio 👉 Division: www.chalmers.se/Indbio 👉 Projects: https://lnkd.in/eH3X6yud  ~~  #industrialbiotechnology #circulareconomy #diversity #phddefense #biobasedmaterials #wood #robustness #biomaterials #upcycling #circularity

NOW ONLINE the first work fully design in my research group of Biochemistry and Industrial Biotechnology at the Dipartimento di Biotecnologie - Università di Verona! Many thanks to the former (Luca Zuliani, Paola Bordoli, and Annalaura Coltro), and current (Luca Bombardi, Andrea Salini) lab members, as well as to our fantastic collaborators (Martina Aullitto, Marco Andreolli, Nicola Vitulo, Claudio Zaccone, and Silvia Lampis). It was both a lot of learning and fun to work together! Ad Maiora Semper! #enzymes #research #biochemistry #circulareconomy #extremophiles #themostableenzymes #biomassvalorization #IJMS  #carbohydrate-active #enzymes; #spentmushroomsubstrate; #microbialdiversity; #microbiomedynamics; #microbiomes; #metagenomicanalyses; #biomassconversion; #biorefineries; #totalcarbohydrateanalysis; #thermalanalysis International Journal of Molecular Sciences MDPI https://lnkd.in/dk7_aYJi

📢 Excited to share my recent research paper titled "Elucidating the Molecular Mechanism of Ultrasound-Enhanced Lipase-Catalyzed Biodiesel Synthesis: A Computational Study," published in the journal Biomass Conversion and Biorefinery by Springer Nature Technology and Publishing Solutions! 📚 In recent years, there's been significant exploration into sono-enzymatic biodiesel synthesis from diverse feedstocks, with a particular focus on the influence of ultrasound (US) on lipase structures. However, the vital connections between structural changes induced by US and their resultant activity enhancement remained elusive. Our study aims to bridge this gap by shedding light on the intricate molecular mechanisms underlying the amplified lipase-catalyzed reactions in biodiesel synthesis due to ultrasound. Through extensive molecular simulations, we dissected the 3D structures and amino acid compositions of the binding pockets of two prominent lipases - CALB and TLL. Our investigation delved into docking analysis of various ligands, encompassing both substrates and reaction products, to uncover the interacting amino acid residues. Importantly, we identified how ultrasound induces changes in the secondary structure of lipases, a phenomenon documented in previous research. One of our key discoveries highlighted that many binding pockets are strategically located within the α-helix and random coil motifs, with binding interactions primarily driven by hydrogen bonding and hydrophobic forces. Intriguingly, the application of ultrasound led to an expansion of the α-helix and random coil regions in lipases, effectively enlarging the catalytic cavity. This, in turn, facilitates easier substrate access to the active site, ultimately resulting in heightened enzymatic activity and faster reaction kinetics, yielding higher product yields. Our findings underscore the incredible potential of combining ultrasound with enzymatic catalysis, a fusion that holds promise for more efficient and sustainable biodiesel synthesis. 🌱💡 For more details, feel free to check out the full paper in Biomass Conversion and Biorefinery (URL: https://lnkd.in/dYrkt3y4). Looking forward to further exploring the exciting realms at the intersection of ultrasound and enzyme-driven processes! 🚀 #Research #EnzymeCatalysis #Biodiesel #UltrasoundEnhancement #MolecularMechanisms #ScienceAtWork #announcement #phd #phdlife

I'm glad to share with you our latest scientific product: an origial article published in Journal of Chemical Technology and Biotechnology 🎊🍾🎉.The article is “Intensification of multiphase systems with a Jet-loop reactor: characterization of mass transfer, mixing time, drop oil size and biomass growth” by Paola Gabriela Bourguett Rosas, Mario Alfonso Arenas-García, Berenice Vergara, Claudia Guerrero-Barajas and Alberto Ordaz. The development of processes for transforming vegetable oils into useful products has received increasing attention in the biotechnological field. The reactor used for this purpose should meet adequate mass transfer, mixing, and oil dispersion conditions. In this work, comprehensive research into the use of a jet-loop reactor as a potential alternative to airlift and stirred tank reactors was conducted in the presence of avocado oil in a water-air system. Additionally, the assessment of the jet-loop for biomass growth in the presence of oil was performed. The results showed that the jet-loop reactor presented superior performance in most cases compared to the stirred tank and airlift reactors. Particularly, the jet-loop exhibits an interesting potential to reduce the size of the avocado oil droplets, which could lead to a significative increase in the growth rate of Wautersia eutropha. We are sure you will enjoy this article: https://lnkd.in/gBfKNWQT 😉 👌 🎊🎊 Grupo de Investigación de biomanufactura sistémica, Departamento de Bioingeniería, Campus Estado de México, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey

I am thrilled to announce the publication of my first research article titled "Elucidating the molecular mechanism of ultrasound-enhanced lipase-catalyzed biodiesel synthesis: A computational study" published in journal Biomass conversion and Biorefinery by Springer Nature Technology and Publishing Solutions Recent years have seen a growing interest in using ultrasound for biodiesel production from various sources. Our study focuses on lipase enzymes and their response to ultrasound used advanced simulations to reveal how ultrasound-induced structural changes improve the enzymes' catalytic activity. The study highlighted the role of specific structural elements in binding pockets and the widening of catalytic cavities due to sonication. This led to better substrate access, resulting in improved enzymatic activity, faster reactions, and higher biodiesel yields. The research opens new paths for refining biodiesel production through interdisciplinary efforts. I would like to express my gratitude to our mentor Karan Kumar sir for his guidance throughout, without him this would have not been possible. I would also like to thank my colleagues Pallavi Patro and Varsha Y. for their support and teamwork. Below is the link for the article: https://lnkd.in/dSNXhuyP #Biology #Bioinformatics #MolecularBiology