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Early immune responses and parasite tissue distribution in mice experimentally infected with oocysts of either archetypal or non-archetypal genotypes of Toxoplasma gondii

Published online by Cambridge University Press:  14 December 2020

Daniela P. Chiebao Open the ORCID record for Daniela P. Chiebao [Opens in a new window] ,
Paul M. Bartley Open the ORCID record for Paul M. Bartley [Opens in a new window] ,
Francesca Chianini ,
Lauren E. Black ,
Alison Burrells ,
Hilda F. J. Pena ,
Rodrigo M. Soares ,
Elisabeth A. Innes  and
Frank Katzer Open the ORCID record for Frank Katzer [Opens in a new window]
Daniela P. Chiebao*
Affiliation:
Department of Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Animal Science – FMVZ, University of Sao Paulo, 87 Professor Doutor Orlando Marques de Paiva Avenue, 05508-270São Paulo, Brazil
Paul M. Bartley
Affiliation:
Moredun Research Institute, Pentland Science Park, Bush Loan, EdinburghEH26 0PZ, UK
Francesca Chianini
Affiliation:
Moredun Research Institute, Pentland Science Park, Bush Loan, EdinburghEH26 0PZ, UK
Lauren E. Black
Affiliation:
Moredun Research Institute, Pentland Science Park, Bush Loan, EdinburghEH26 0PZ, UK
Alison Burrells
Affiliation:
Moredun Research Institute, Pentland Science Park, Bush Loan, EdinburghEH26 0PZ, UK
Hilda F. J. Pena
Affiliation:
Department of Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Animal Science – FMVZ, University of Sao Paulo, 87 Professor Doutor Orlando Marques de Paiva Avenue, 05508-270São Paulo, Brazil
Rodrigo M. Soares
Affiliation:
Department of Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Animal Science – FMVZ, University of Sao Paulo, 87 Professor Doutor Orlando Marques de Paiva Avenue, 05508-270São Paulo, Brazil
Elisabeth A. Innes
Affiliation:
Moredun Research Institute, Pentland Science Park, Bush Loan, EdinburghEH26 0PZ, UK
Frank Katzer
Affiliation:
Moredun Research Institute, Pentland Science Park, Bush Loan, EdinburghEH26 0PZ, UK
*
Author for correspondence: Daniela P. Chiebao, E-mail: daniela.chiebao@sp.gov.br
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Abstract

In most of the world Toxoplasma gondii is comprised of archetypal types (types I, II and III); however, South America displays several non-archetypal strains. This study used an experimental mouse model to characterize the immune response and parasite kinetics following infection with different parasite genotypes. An oral inoculation of 50 oocysts per mouse from T. gondii M4 type II (archetypal, avirulent), BrI or BrIII (non-archetypal, virulent and intermediate virulent, respectively) for groups (G)2, G3 and G4, respectively was used. The levels of mRNA expression of cytokines, immune compounds, cell surface markers and receptor adapters [interferon gamma (IFNγ), interleukin (IL)-12, CD8, CD4, CD25, CXCR3 and MyD88] were quantified by SYBR green reverse transcription-quantitative polymerase chain reaction. Lesions were characterized by histology and detection by immunohistochemistry established distribution of parasites. Infection in G2 mice was mild and characterized by an early MyD88-dependent pathway. In G3, there were high levels of expression of pro-inflammatory cytokines IFNγ and IL-12 in the mice showing severe clinical symptoms at 8–11 days post infection (dpi), combined with the upregulation of CD25, abundant tachyzoites and tissue lesions in livers, lungs and intestines. Significant longer expression of IFNγ and IL-12 genes, with other Th1-balanced immune responses, such as increased levels of CXCR3 and MyD88 in G4, resulted in survival of mice and chronic toxoplasmosis, with the occurrence of tissue cysts in brain and lungs, at 14 and 21 dpi. Different immune responses and kinetics of gene expression appear to be elicited by the different strains and non-archetypal parasites demonstrated higher virulence.

Keywords

CytokinesMyD88non-archetypal strainsT cellstoxoplasmosisvirulence
Type
Research Article
Information
Parasitology , Volume 148 , Issue 4 , April 2021 , pp. 464 - 476
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Copyright © The Author(s) 2020. Published by Cambridge University Press

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