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Comparative Embryo Culture pp 93–103Cite as

In Vitro Maturation, Fertilization, and Culture of Pig Oocytes and Embryos

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2006))

Abstract

Assisted reproductive technologies in the pig are critical for the production of genetically modified pigs as models of human disease and to improve production agriculture. Methods of oocyte maturation, fertilization, and culture all play an extremely important role in how the embryo, fetus, and offspring will develop. In this chapter, we discuss the historical methods and recent advances that have been essential in promoting efficient and competent embryo development. Here we describe the procedures that can be used to mature, fertilize, and culture pig embryos to the blastocyst stage.

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Acknowledgments

Funding from Food for the 21st Century and the National Institutes of Health are acknowledged (U42 OD011140, R01 HD 080636).

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Authors and Affiliations

  1. Division of Animal Sciences, University of Missouri, Columbia, MO, USA

    Bethany K. Redel, Lee D. Spate & Randall S. Prather

  2. National Swine Resource and Research Center, University of Missouri, Columbia, MO, USA

    Bethany K. Redel, Lee D. Spate & Randall S. Prather

Authors
  1. Bethany K. Redel
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  2. Lee D. Spate
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  3. Randall S. Prather
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Corresponding author

Correspondence to Bethany K. Redel .

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Editors and Affiliations

  1. Department of Reproductive Sciences, Omaha’s Henry Doorly Zoo and Aquarium, Omaha, NE, USA

    Jason R. Herrick

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Redel, B.K., Spate, L.D., Prather, R.S. (2019). In Vitro Maturation, Fertilization, and Culture of Pig Oocytes and Embryos. In: Herrick, J. (eds) Comparative Embryo Culture. Methods in Molecular Biology, vol 2006. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9566-0_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9566-0_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9565-3

  • Online ISBN: 978-1-4939-9566-0

  • eBook Packages: Springer Protocols

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