Abstract
The monitoring of specific molecules in the living body has historically required sample removal (e.g., blood draws, microdialysis) followed by analysis via cumbersome, laboratory-bound processes. Those few exceptions to this rule (e.g., glucose, pyruvate, the monoamines) are monitored using “one-off” technologies reliant on the specific enzymatic or redox reactivity of their targets, and thus not generalizable to the measurement of other targets. In response we have developed in vivo electrochemical aptamer-based (E-AB) sensors, a modular, receptor-based measurement technology that is independent of the chemical reactivity of its targets, and thus has the potential to be generalizable to a wide range of analytes. To further the adoption of this in vivo molecular measurement approach by other researchers and to accelerate its ultimate translation to the clinic, we present here our standard protocols for the fabrication and use of intravenous E-AB sensors.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Xiao Y, Lubin AA, Heeger AJ, Plaxco KW (2005) Angew Chem Int Ed 44:5456–5459
Xiao Y, Piorek BD, Plaxco KW, Heeger AJJ (2005) Am Chem Soc 127:17990–17991
Dauphin-Ducharme P, Plaxco KW (2016) Anal Chem 88:11654–11662
White RJ, Phares N, Lubin AA, Xiao Y, Plaxco KW (2008) Langmuir 24:10513–10518
Arroyo-Currás N, Dauphin-Ducharme P, Ortega G, Ploense KL, Kippin TE, Plaxco KW (2018) ACS Sensors 3:360–366
Schoukroun-Barnes LR, Macazo FC, Gutierrez B, Lottermoser J, Liu J, White RJ (2016) Annu Rev Anal Chem 9:163–181
Lubin AA, Plaxco KW (2010) Acc Chem Res 43:496–505
Arroyo-Currás N, Ortega G, Copp DA, Ploense KL, Plaxco ZA, Kippin TE, Hespanha JP, Plaxco KW (2018) ACS Pharmacol Transl Sci 1:110–118
Arroyo-Currás N, Somerson J, Vieira PA, Ploense KL, Kippin TE, Plaxco KW (2017) Proc Natl Acad Sci U S A 114:645–650
Dauphin-Ducharme P, Yang K, Arroyo-Currás N, Ploense KL, Zhang Y, Gerson J, Kurnik M, Kippin TE, Stojanovic MN, Plaxco KW (2019) ACS Sensors 4:2832–2837
Ferguson BS, Hoggarth DA, Maliniak D, Ploense K, White RJ, Woodward N, Hsieh K, Bonham AJ, Eisenstein M, Kippin TE, Plaxco KW, Soh HT (2013) Sci Transl Med 5:213ra165
Mage PL, Ferguson BS, Maliniak D, Ploense KL, Kippin TE, Soh HT (2017) Nat Biomed Eng 1:0070
Idili A, Arroyo-Currás N, Ploense KL, Csordas AT, Kuwahara M, Kippin TE, Plaxco KW (2019) Chem Sci 10:8164–8170
Li H, Li S, Dai J, Li C, Zhu M, Li H, Lou X, Xia F, Plaxco KW (2019) Chem Sci 10:10843–10848
Taylor IM, Du Z, Bigelow ET, Eles JR, Horner AR, Catt KA, Weber SG, Jamieson BG, Cui XT (2017) J Mater Chem B 5:8417–8417
Curtis SD, Ploense KL, Kurnik M, Ortega G, Parolo C, Kippin TE, Plaxco KW, Arroyo-Currás N (2019) Anal Chem 91:12321–12328
Arroyo-Currás N, Scida K, Ploense KL, Kippin TE, Plaxco KW (2017) Anal Chem 89:12185–12191
Trasatti S, Petrii OA (1991) Pure Appl Chem 63:711–734
White RJ, Rowe AA, Plaxco KW (2010) Analyst 135:589–594
White RJ, Plaxco KW (2010) Anal Chem 82:73–76
Vieira PA, Shin CB, Arroyo-Currás N, Ortega G, Li W, Keller AA, Plaxco KW, Kippin TE (2019) Front Mol Biosci 6:69
National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals (2010) Guide for the care and use of laboratory animals. National Academies Press
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Dauphin-Ducharme, P., Ploense, K.L., Arroyo-Currás, N., Kippin, T.E., Plaxco, K.W. (2022). Electrochemical Aptamer-Based Sensors: A Platform Approach to High-Frequency Molecular Monitoring In Situ in the Living Body. In: Ossandon, M.R., Baker, H., Rasooly, A. (eds) Biomedical Engineering Technologies. Methods in Molecular Biology, vol 2393. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1803-5_25
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1803-5_25
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1802-8
Online ISBN: 978-1-0716-1803-5
eBook Packages: Springer Protocols